Antibacterial activity of Aloe vera extract and chitosan nanoparticles against Porphyromonas gingivalis and fibroblast viability assay analysis

Pratidina Fitri Ramadhani; Masulili, Sri Lelyati C; Robert Lessang; Yuniarti Soeroso; Natalina; Ette Soraya Tadjoedin; Padmini Hari

doi:10.51094/jxiv.368

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Pratidina Fitri Ramadhani University of Indonesia, Faculty of dentistry, Residency of Periodontology
Masulili, Sri Lelyati C University of Indonesia, Faculty of dentistry, Department of Periodontology
Robert Lessang University of Indonesia, Faculty of dentistry, Department of Periodontology
Yuniarti Soeroso University of Indonesia, Faculty of dentistry, Department of Periodontology
Natalina University of Indonesia, Faculty of dentistry, Department of Periodontology
Ette Soraya Tadjoedin University of Indonesia, Faculty of dentistry, Department of Periodontology
Padmini Hari MAHSA University, Faculty of Dentistry

DOI:

https://doi.org/10.51094/jxiv.368

キーワード:

antibacterial activity、 aloe vera extract、 chitosan nanoparticles、 porphyromonas gingivalis、 fibroblast cell viability

抄録

The use of herbal medicine as an alternative therapy to reduce periodontal inflammation is then being developed to resolve antibiotic resistance. Aloe vera is receiving much scientific attention, but herbal drug formulation has limitations. The efficiency of herbal medicines can improve with a drug delivery system utilizing chitosan nanoparticles. This research aims to develop a potential antibiotic combining Aloe vera extract and chitosan nanoparticles. Evaluate their antibacterial activity against P.gingivalis and toxicity on BHK-21 fibroblast cells. Determining Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) values used the microdilution method in 96 well plates. ELISA did a viability assay. The MIC and MBC were obtained in concentrations of 80% Aloe vera – 25% chitosan nanoparticles. A combination of 80% Aloe vera extract and various chitosan nanoparticle formulations had a greater inhibitory zone than a single sample used. Combining these two materials showed potent antibacterial activity and was not toxic.

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no conflict of interests regarding the publication of this article.

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