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Antibacterial activity of Aloe vera extract and chitosan nanoparticles against Porphyromonas gingivalis and fibroblast viability assay analysis

##article.authors##

  • Pratidina Fitri Ramadhani University of Indonesia, Faculty of dentistry, Residency of Periodontology
  • Masulili, Sri Lelyati C University of Indonesia, Faculty of dentistry, Department of Periodontology 
  • Robert Lessang University of Indonesia, Faculty of dentistry, Department of Periodontology 
  • Yuniarti Soeroso University of Indonesia, Faculty of dentistry, Department of Periodontology 
  • Natalina University of Indonesia, Faculty of dentistry, Department of Periodontology 
  • Ette Soraya Tadjoedin University of Indonesia, Faculty of dentistry, Department of Periodontology 
  • Padmini Hari MAHSA University, Faculty of Dentistry

DOI:

https://doi.org/10.51094/jxiv.368

キーワード:

antibacterial activity、 aloe vera extract、 chitosan nanoparticles、 porphyromonas gingivalis、 fibroblast cell viability

抄録

The use of herbal medicine as an alternative therapy to reduce periodontal inflammation is then being developed to resolve antibiotic resistance. Aloe vera is receiving much scientific attention, but herbal drug formulation has limitations. The efficiency of herbal medicines can improve with a drug delivery system utilizing chitosan nanoparticles. This research aims to develop a potential antibiotic combining Aloe vera extract and chitosan nanoparticles. Evaluate their antibacterial activity against P.gingivalis and toxicity on BHK-21 fibroblast cells. Determining Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) values used the microdilution method in 96 well plates. ELISA did a viability assay. The MIC and MBC were obtained in concentrations of 80% Aloe vera – 25% chitosan nanoparticles. A combination of 80% Aloe vera extract and various chitosan nanoparticle formulations had a greater inhibitory zone than a single sample used. Combining these two materials showed potent antibacterial activity and was not toxic. 

利益相反に関する開示

no conflict of interests regarding the publication of this article.

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引用文献

Mombelli A, Samaranayake L. Topical and systemic antibiotics in the management of periodontal diseases. International Dental Journal 2004; 54: 3–14.

Lestari E, Severin J, Verbrugh H. Antimicrobial Resistance Among Pathogenic Bacteria in Southeast Asia.

Southeast Asian J Trop Med Public Health 2012; 43: 8-15.

Saraf A.S. Applications of novel drug delivery system for herbal formulations. Fitoterapia 2010; 81: 680–689.

Subbiah U, Elango S, Jayesh R. Herbals and green synthesized nanoparticles in dentistry. Nanobiomaterials in Clinical Dentistry 2019; 10: 617–646.

Cheung W.H, Szeto Y. S, McKay G. Enhancing the adsorption capacities of acid dyes by chitosan nano particles. Bioresource Technology 2009; 100: 1143–1148.

Mohanraj V. J, Chen Y. Nanoparticles - A review. Tropical Journal of Pharmaceutical Research 2006; 5: 561-573.

Rafiei M, Kiani F, Sayehmiri F. Study of Porphyromonas gingivalis in periodontal diseases: A systematic review and meta-analysis. Med J Islam Republic Iran 2017; 31: 355–362.

Khare T, Anand U, Dey A, Assaraf Y.G, Chen Z, Liu Z. Exploring Phytochemicals for Combating Antibiotic

Resistance in Microbial Pathogens. Front Pharmacol J 2021; 12: 720-726

Mercado N, Bhatt P, Sutariya V, Florez F, Pathak Y. Application of Nanoparticles in Treating Periodontitis:

Preclinical and Clinical Overview in Surface Modification of Nanoparticles for Targeted Drug Delivery. Springer

Andres Y, Giraud L, Gerente C, Le Cloirec P. Antibacterial Effects of Chitosan Powder: Mechanisms of Action. Environmental Technology 2007; 28: 1357–1363.

Sánchez M, González-Burgos E, Iglesias I, Gómez-Serranillos M. Pharmacological Update Properties of Aloe Vera and its Major Active Constituents. Molecules 2020; 25: 13-24.

Xu Y, Du Y. Effect of molecular structure of chitosan on protein delivery properties of chitosan nanoparticles. International Journal of Pharmaceutics 2003; 250: 215–226 International Publishing 2019; 5: 467-480.

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投稿日時: 2023-04-20 17:31:23 UTC

公開日時: 2023-04-28 00:41:40 UTC
研究分野
歯学