Preprint / Version 1

Antibacterial activity of Aloe vera extract and chitosan nanoparticles against Porphyromonas gingivalis and fibroblast viability assay analysis

##article.authors##

  • Pratidina Fitri Ramadhani University of Indonesia, Faculty of dentistry, Residency of Periodontology
  • Sri Lelyati C Masulili University of Indonesia, Faculty of dentistry, Department of Periodontology 
  • Robert Lessang University of Indonesia, Faculty of dentistry, Department of Periodontology 
  • Yuniarti Soeroso University of Indonesia, Faculty of dentistry, Department of Periodontology 
  • Natalina University of Indonesia, Faculty of dentistry, Department of Periodontology 
  • Ette Soraya Tadjoedin University of Indonesia, Faculty of dentistry, Department of Periodontology 
  • Padmini Hari MAHSA University, Faculty of Dentistry

DOI:

https://doi.org/10.51094/jxiv.368

Keywords:

antibacterial activity, aloe vera extract, chitosan nanoparticles, porphyromonas gingivalis, fibroblast cell viability

Abstract

The use of herbal medicine as an alternative therapy to reduce periodontal inflammation is then being developed to resolve antibiotic resistance. Aloe vera is receiving much scientific attention, but herbal drug formulation has limitations. The efficiency of herbal medicines can improve with a drug delivery system utilizing chitosan nanoparticles. This research aims to develop a potential antibiotic combining Aloe vera extract and chitosan nanoparticles. Evaluate their antibacterial activity against P.gingivalis and toxicity on BHK-21 fibroblast cells. Determining Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) values used the microdilution method in 96 well plates. ELISA did a viability assay. The MIC and MBC were obtained in concentrations of 80% Aloe vera – 25% chitosan nanoparticles. A combination of 80% Aloe vera extract and various chitosan nanoparticle formulations had a greater inhibitory zone than a single sample used. Combining these two materials showed potent antibacterial activity and was not toxic. 

Conflicts of Interest Disclosure

no conflict of interests regarding the publication of this article.

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Submitted: 2023-04-20 17:31:23 UTC

Published: 2023-04-28 00:41:40 UTC
Section
Dentistry