The Reinstatement of the Genus Kloeckeraspora Niehaus (1932) (Apiculate Yeast)

抄録

The genus Kloeckeraspora Niehaus was introduced with Kloeckerasora osmophila Niehaus. However, the genus has been neither accepted nor recognized for a long time due to the unclear generic concept. In the phylogeneetic tree based on the 26S rRNA gene D1/D2 domain sequences derived from the neighbour-joining method for the seven species, the three species assigned to the genus Kloeckeraspora represented an extremely long branch to the Hanseniaspora species. The calculated pair-wise sequence similarities were extremely low (86.2 - 88.5%). Incidentally, the sequence similarity between Vanderwatozyma polyspora and Saccharomyces cerevisiae used as reference standards was 94.0%. Within the respective genus, the similarities were 93.6 - 98.5% in the genus Kloeckeraspora and 96.3 - 99.1% in the genus Hanseniaspora. From the experimental data, the genus Kloeckeraspora should be unequivocally accepted phylogenetically and taxonomically. However, the two genera were not yet perfectly taxonomic homogeneous-natured, since there were a few species showing below 98% sequence similarity and longer phylogenetic branches in the two genera Kloeckeraspora and Hanseniaspora.