The Reinstatement of the Genus Kloeckeraspora Niehaus (1932) (Apiculate Yeast)

Taweesak Malimas; Huong Thi Lan Vu; Pattaraporn Yukphan; Somboon Tanasupawat; Yuzo Yamada

doi:10.51094/jxiv.317

##article.authors##

Taweesak Malimas Microbial Laboratory Biosafety Level-1
Huong Thi Lan Vu University of Science, Vietnam National University-HCM City
Pattaraporn Yukphan BIOTEC Culture Collection (BCC), National Center for Genetic Engineering and Biotechnology (BIOTEC)
Somboon Tanasupawat Faculty of Pharmaceutical Sciences, Chulalongkorn University
Yuzo Yamada Faculty of Agriculture, Shizuoka University

DOI:

https://doi.org/10.51094/jxiv.317

Keywords:

Kloeckeraspora osmophila, Kloeckerasora vineae, Kloeckeraspora occidentalis, Hanseniaspora valvyensis, Hanseniaspora uvarum

Abstract

The genus Kloeckeraspora Niehaus was introduced with Kloeckerasora osmophila Niehaus. However, the genus has been neither accepted nor recognized for a long time due to the unclear generic concept. In the phylogeneetic tree based on the 26S rRNA gene D1/D2 domain sequences derived from the neighbour-joining method for the seven species, the three species assigned to the genus Kloeckeraspora represented an extremely long branch to the Hanseniaspora species. The calculated pair-wise sequence similarities were extremely low (86.2 - 88.5%). Incidentally, the sequence similarity between Vanderwatozyma polyspora and Saccharomyces cerevisiae used as reference standards was 94.0%. Within the respective genus, the similarities were 93.6 - 98.5% in the genus Kloeckeraspora and 96.3 - 99.1% in the genus Hanseniaspora. From the experimental data, the genus Kloeckeraspora should be unequivocally accepted phylogenetically and taxonomically. However, the two genera were not yet perfectly taxonomic homogeneous-natured, since there were a few species showing below 98% sequence similarity and longer phylogenetic branches in the two genera Kloeckeraspora and Hanseniaspora.

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Author Biographies

Taweesak Malimas, Microbial Laboratory Biosafety Level-1

He is a research scientist.

Huong Thi Lan Vu, University of Science, Vietnam National University-HCM City

Department of Microbiology, Faculty of Biology and Biotechnology, University of Science, Vietnam National University-HCM City

Pattaraporn Yukphan, BIOTEC Culture Collection (BCC), National Center for Genetic Engineering and Biotechnology (BIOTEC)

She is a research staff at BIOTEC.

Somboon Tanasupawat, Faculty of Pharmaceutical Sciences, Chulalongkorn University

He is a professor.

Yuzo Yamada, Faculty of Agriculture, Shizuoka University

Laboratory of Applied Microbiology (Professor Emeritus), Department of Agricultural Chemistry, Faculty of Agriculture, Shizuoka University

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