Preprint / Version 2

The Reinstatement of the Genus Kloeckeraspora Niehaus (1932) (Apiculate Yeast)

##article.authors##

  • Taweesak Malimas Microbial Laboratory Biosafety Level-1
  • Huong Thi Lan Vu University of Science, Vietnam National University-HCM City
  • Pattaraporn Yukphan BIOTEC Culture Collection (BCC), National Center for Genetic Engineering and Biotechnology (BIOTEC)
  • Somboon Tanasupawat Faculty of Pharmaceutical Sciences, Chulalongkorn University
  • Yuzo Yamada Faculty of Agriculture, Shizuoka University

DOI:

https://doi.org/10.51094/jxiv.317

Keywords:

Kloeckeraspora osmophila, Kloeckerasora vineae, Kloeckeraspora occidentalis, Hanseniaspora valvyensis, Hanseniaspora uvarum

Abstract

The genus Kloeckeraspora Niehaus was introduced with Kloeckerasora osmophila Niehaus. However, the genus has been neither accepted nor recognized for a long time due to the unclear generic concept. In the phylogeneetic tree based on the 26S rRNA gene D1/D2 domain sequences derived from the neighbour-joining method for the seven species, the three species assigned to the genus Kloeckeraspora represented an extremely long branch to the Hanseniaspora species. The calculated pair-wise sequence similarities were extremely low (86.2 - 88.5%). Incidentally, the sequence similarity between Vanderwatozyma polyspora and Saccharomyces cerevisiae used as reference standards was 94.0%. Within the respective genus, the similarities were 93.6 - 98.5% in the genus Kloeckeraspora and 96.3 - 99.1% in the genus Hanseniaspora. From the experimental data, the genus Kloeckeraspora should be unequivocally accepted phylogenetically and taxonomically. However, the two genera were not yet perfectly taxonomic homogeneous-natured, since there were a few species showing below 98% sequence similarity and longer phylogenetic branches in the two genera Kloeckeraspora and Hanseniaspora.

 

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Author Biographies

Taweesak Malimas, Microbial Laboratory Biosafety Level-1

He is a research scientist.

Huong Thi Lan Vu, University of Science, Vietnam National University-HCM City

Department of Microbiology, Faculty of Biology and Biotechnology, University of Science, Vietnam National University-HCM City

Pattaraporn Yukphan, BIOTEC Culture Collection (BCC), National Center for Genetic Engineering and Biotechnology (BIOTEC)

She is a research staff at BIOTEC.

Somboon Tanasupawat, Faculty of Pharmaceutical Sciences, Chulalongkorn University

He is a professor.

Yuzo Yamada, Faculty of Agriculture, Shizuoka University

Laboratory of Applied Microbiology (Professor Emeritus), Department of Agricultural Chemistry, Faculty of Agriculture, Shizuoka University

References

Cadez, N. and Smith, M.Th. (2011) Hanseniaspora Zikes (1912) In: Kurtzman, C.P., Fell, J.W. and Boekhout, T. (ed). The Yeasts: A Taxonomic Study, 5th edition, vol. 2. London: Elsevier, p. 421-434.

Kurtzman, C.P. (2003) Phylogenetic circumscription of Saccharomyces, Kluyveromyces and other members of the Saccharomycetaceae and the proposal of the new genera Lachancea, Nakaseomyces, Naumovia, Vanderwaltozyma and Zygotorulaspora. FFEMS Yeast Research 4: 233-245.

Lachance, M.A. (2012) In defense of sxual life cycles: The forma asexualis - an informal proposal. Yeast Newsletter 61: 24-25.

Malimas, T., Vu, H.T.L., Yukphan, P., Tanasupwat, S. and Yamada, Y. (2023) The subdivision of the genus Eremolthecum Borzi emend. Kurzman. Jxiv (DOI: https://doi.org/10.51094/jxiv.285).

Niehaus, C.J.G. (1932) Utersuchungen über Apiculatushefen. Zentralbl. Bakteriol. Parasitenk. Infektionskrankh. Abt. II, 87: 97-150.

Vu, H.T.L., Yukphan, P., Tanasupawat, S., Mikata, K. and Yamada, Y. (2022a) The revision of Schizosacchromycetaceae. Jxiv (DOI: https://doi.org/10.51094/jxiv.188).

Vu, H.T.L., Yukphan, P., Tanasupawat, S. and Yamada, Y. (2022b) The generic circumscription of Kockiozyma (Lipomycetaceae). Jxiv (DOI: https://doi.org/10.51094/jxiv.221).

Yamada, Y., Maeda, K. and Banno, I. (1992a) The phylogenetic relationships of the Q6-equipped species in the teleomorphic apiculate yeast genera Hanseniaspora, Nadsonia and Saccharomycodes based on the partial sequences of 18S and 26S ribosomal ribonucleic acids. J. Gen. Appl. Microbiol. 38: 585-596.

Yamada, Y., Maeda, K. and Banno, I. (1992b) An emendation of Kloeckeraspora Nihaus with the type species, Kloeckeraspora osmophila and the proposals of the two new combinations, Kloeckerasora occidentallis and Kloeckeraspora vineae (Sacchro-mycetaceae). Bull. JFCC, 8: 79-85.

Yamada, Y., Vu, H.T.L, Yukphan, P. and Tanasupawat, S. (2022) The revision of Lipomycetaceae. Jxiv (DOI: https://doi.org/10.51094//jxiv.189).

Yamada, Y. (2023) The generic circumscription of Eremothecium emend. Kurtzma, Jxiv (DOI: https://doi.org/1051094//jxiv.270)

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Submitted: 2023-02-28 01:19:56 UTC

Published: 2023-02-28 23:43:32 UTC — Updated on 2023-03-13 00:35:06 UTC

Versions

Reason(s) for revision

In the genus Hanseniaspora, a new taxonomic subgroup was found, designated as subgroup b-3. Therefore, the taxonomic heterogeneous-natures of the genus were increased. However, the subdivision of the genus may be difficult. In Appendix, the subgoup b-3 was mentioned. 
Section
Biology, Life Sciences & Basic Medicine