High- Polyamines Yeast Strain with Autophagy-Inducing Ability
DOI:
https://doi.org/10.51094/jxiv.1614Keywords:
polyamine, spermidine, yeast, autophagy, anti-aging, fermentation, non-GMOAbstract
Polyamines are essential substances for cellular life activities, including cell proliferation. In recent years, polyamines have been reported to promote autophagy and extend lifespan, garnering attention as anti-aging materials. Foods rich in polyamines include natto, cheese, and mushrooms; however, they do not provide enough amounts to supplement the declining levels of polyamines in the body with aging. Therefore, there is a demand for food ingredients containing polyamines at high concentrations. We treated the Saccharomyces cerevisiae strain with UV mutation and developed the 3L63 strain, which contains a high level of spermine. Surprisingly, this polyamine-enriched yeast contains 5.9 times more polyamines than the parent strain. Furthermore, the 3L63 strain features a high content of spermine, which has a high absorption rate in the body among polyamines. The 3L63 strain has been successfully produced on an industrial scale of up to 10kL, and all the obtained dried yeast powders (Fermeast SP, Fermeast SP100) met all food safety standards. Functional assays in vitro demonstrated that Elion SP100 promotes the proliferation of human dermal fibroblasts. Moreover, this effect was also confirmed in aging-induced cells. Additionally, it was verified that Elion SP100 promotes autophagy in HeLa cells, surpassing the results of spermine, which is known as an autophagy activator. This study demonstrated the development of a non-genetically modified high polyamine yeast strain as a functional food component and assessed the potential of its functional ingredients.
Conflicts of Interest Disclosure
All authors are employees of Mitsubishi Gas Chemical Company, Inc. All funding for this research was provided by Mitsubishi Gas Chemical Company, Inc.Downloads *Displays the aggregated results up to the previous day.
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Submitted: 2025-10-07 23:14:08 UTC
Published: 2025-10-21 01:00:29 UTC
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Tomoyo Koshizawa
Tomoe Numaguchi
Masanori Tamakoshi
Yuuki Sato
Katsuyuki Hashimoto
Mohamad Ishak, Nur Syafiqah
Kazuto Ikemoto
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