Fabrication of a cell culture scaffold that mimics the composition and structure of bone marrow extracellular matrix
DOI:
https://doi.org/10.51094/jxiv.583Keywords:
decellularized fetal bone marrow, extracellular matrix, cell culture scaffoldAbstract
Cell culture models that mimic tissue environments are useful for cell and extracellular matrix (ECM) function analysis. Decellularized tissues with tissue-specific ECM are expected to be applied as cell culture scaffolds, however it is often difficult for seeded cells to permeate their structures. In this study, we evaluated the adhesion and proliferation of mouse fibroblasts seeded onto decellularized bone marrow scaffolds that we fabricated from adult and fetal porcine. Decellularized fetal bone marrow displays more cell attachment and faster cell proliferation than decellularized adult bone marrow. Our findings suggest that decellularized fetal bone marrow is useful as a cell culture scaffold with bone marrow ECM and structure.
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The authors declare no conflicts of interest associated with this manuscript.Downloads *Displays the aggregated results up to the previous day.
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Submitted: 2023-12-23 22:57:59 UTC
Published: 2023-12-27 04:09:20 UTC
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Ayana Yamaguchi
Yoshihide Hashimoto
Jun Negishi
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