A genotyping method for discrimination between Saccharomyces cerevisiae and Saccharomyces paradoxus from natural environment
DOI:
https://doi.org/10.51094/jxiv.1287Keywords:
screening, Saccharomyces cerevisiae, Saccharomyces paradoxusAbstract
In this study, we developed a straightforward technique for differentiating between Saccharomyces cerevisiae and Saccharomyces paradoxus, thereby facilitating the isolation of S. cerevisiae from natural environments. Our findings suggest that this method is easy, quick, and beneficial for producing fermented foods and beverages using wild S. cerevisiae.
Conflicts of Interest Disclosure
The authors declare no conflict of interest. All experiments complied with the current laws of the country, in which they were performed. This study was presented orally at the 69th Annual Meeting of the Mycological Society of Japan (E-12; 18th, May, 2025Downloads *Displays the aggregated results up to the previous day.
References
Dashko, S., Liu, P., Volk, H., Butinar, L., Piškur, J., & Fay, J. C. (2016). Changes in the relative abundance of two Saccharomyces species from oak forests to wine fermentations. Frontiers in Microbiology, 7, 215.
Fernández-Espinar, M. T., Martorell, P., De Llanos, R., & Querol, A. (2006). Molecular methods to identify and characterize yeasts in foods and beverages. Yeasts in Foods and Beverages, 55-82.
Hashim, H. O., & Al-Shuhaib, M. B. S. (2019). Exploring the potential and limitations of PCR-RFLP and PCR-SSCP for SNP detection: A review. Journal of Applied Biotechnology Reports, 6(4), 137-144.
Kitagaki, H., & Kitamoto, K. (2013). Breeding research on sake yeasts in Japan: history, recent technological advances, and future perspectives. Annual Review of Food Science and Technology, 4(1), 215-235.
Nikulin, J., Vidgren, V., Krogerus, K., Magalhães, F., Valkeemäki, S., Kangas-Heiska, T., & Gibson, B. (2020). Brewing potential of the wild yeast species Saccharomyces paradoxus. European Food Research and Technology, 246, 2283-2297.
Sampaio, J. P., & Gonçalves, P. (2008). Natural populations of Saccharomyces kudriavzevii in Portugal are associated with oak bark and are sympatric with S. cerevisiae and S. paradoxus. Applied and Environmental Microbiology, 74(7), 2144-2152.
Sniegowski, P. D., Dombrowski, P. G., & Fingerman, E. (2002). Saccharomyces cerevisiae and Saccharomyces paradoxus coexist in a natural woodland site in North America and display different levels of reproductive isolation from European conspecifics. FEMS Yeast Research, 1(4), 299-306.
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Submitted: 2025-06-02 15:02:58 UTC
Published: 2025-06-04 08:36:12 UTC
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Copyright (c) 2025
Fuka Sawaguchi
Yuki Kobashi
Takashi Kuribayashi
Hina Satone
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
