Discovery of a New Male-sterile Allele, ms1-3, at MALE STERILITY 1 Locus in Cryptomeria japonica
DOI:
https://doi.org/10.51094/jxiv.1241Keywords:
male-sterility, MAS, pollen free, Cryptomeria japonica, pollinosisAbstract
Recently, a candidate gene (CJt020762) for MALE STERILITY 1 (MS1) was identified in Cryptomeria japonica, and two male-sterility alleles (ms1-1 and ms1-2) have been found at the MS1 locus. In this study, we developed a PCR primer set (DNA markers) that amplifies the entire coding sequence of CJt020762 including introns and identifies differences in the length of the amplified fragments. We also conducted marker-assisted selection (MAS) on 1,500 breeding materials collected throughout Japan. As a result, we selected two heterozygous trees with a new four-nucleotide deletion mutation in the coding sequence in Iwate Prefecture. We named this new male-sterility allele ms1-3. Furthermore, artificial crossing revealed that individuals carrying both ms1-1 and ms1-3 (ms1-1/ms1-3) were male-sterile, indicating that ms1-3 can be used for the breeding of male-sterile C. japonica. Because trees with ms1-3 were found only in Iwate Prefecture, they are probably localized around Iwate Prefecture. In addition, it was shown that mutations occurring within an intron does not cause male-sterility.
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Submitted: 2025-05-05 00:14:50 UTC
Published: 2025-05-09 09:08:07 UTC
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Masahiro Watanabe
Yutaka Takeishi
Saneyoshi Ueno
Yoichi Hasegawa
Yoshinari Moriguchi
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