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Methyl syringate mono-glucoside is a crucial intermediate in leptosperin biosynthesis in Leptospermum scoparium (manuka)

##article.authors##

  • 加藤, 陽二 University of Hyogo, School of Human Science and Environment https://researchmap.jp/read0056569
  • 古谷, 優夏 University of Hyogo, School of Human Science and Environment
  • 中井, 逸斗 University of Hyogo, School of Human Science and Environment
  • 高岡, 瑛未 University of Hyogo, School of Human Science and Environment
  • 神里, えみり University of Hyogo, School of Human Science and Environment
  • 丹羽, 利夫 Shubun University, Faculty of Health and Nutrition

DOI:

https://doi.org/10.51094/jxiv.856

キーワード:

Mānuka honey、 Leptosperin、 Leptospermum scoparium、 Methyl syringate glucosides、 Biosynthesis、 Certification

抄録

Leptosperin (methyl syringate-4-O-β-D-gentiobioside) serves as a unique marker for mānuka honey, derived from the manuka plant (Leptospermum scoparium). Despite its importance, the biosynthesis pathway of leptosperin remains unreported. This study investigates the molecular mechanism of leptosperin formation from its aglycone, methyl syringate (MSYR), in manuka plants. Methyl syringate-4-O-β-D-glucopyranoside (MSYR-glucose) was identified in manuka flower nectar but not in mānuka honey. MSYR was distributed in the flowers, leaves, branches, and roots of manuka plants, while MSYR-glucose and leptosperin were only observed in the flowers. By immersing a cut flowering branch in a deuterium-labeled aqueous medium, the formation of deuterated leptosperin (leptosperin-d6) and MSYR-glucose (MSYR-d6-glucose) was analyzed. When MSYR-d6 was added, both MSYR-d6-glucose and leptosperin-d6 were detected. Supplementation with synthetic MSYR-d6-glucose also generated leptosperin-d6, indicating that gentiobioside moiety in leptosperin forms through the conjugation of MSYR with D-glucose, followed by the addition of another D-glucose.

利益相反に関する開示

The authors report no conflicts of interest related to this study.

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投稿日時: 2024-08-21 07:38:21 UTC

公開日時: 2024-08-23 06:20:01 UTC

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Copyright（c）2024 加藤, 陽二
古谷, 優夏
中井, 逸斗
高岡, 瑛未
神里, えみり
丹羽, 利夫

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