Fabrication of a cell culture scaffold that mimics the composition and structure of bone marrow extracellular matrix

Yamaguchi, Ayana; Hashimoto, Yoshihide; Negishi, Jun

doi:10.51094/jxiv.583

##article.authors##

Yamaguchi, Ayana Department of Textile Science and Technology, Graduate School of Science and Technology, Shinshu University
Hashimoto, Yoshihide Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University
Negishi, Jun Department of Textile Science and Technology, Graduate School of Science and Technology, Shinshu University

DOI:

https://doi.org/10.51094/jxiv.583

キーワード:

decellularized fetal bone marrow、 extracellular matrix、 cell culture scaffold

抄録

Cell culture models that mimic tissue environments are useful for cell and extracellular matrix (ECM) function analysis. Decellularized tissues with tissue-specific ECM are expected to be applied as cell culture scaffolds, however it is often difficult for seeded cells to permeate their structures.　In this study, we evaluated the adhesion and proliferation of mouse fibroblasts seeded onto decellularized bone marrow scaffolds that we fabricated from adult and fetal porcine. Decellularized fetal bone marrow displays more cell attachment and faster cell proliferation than decellularized adult bone marrow. Our findings suggest that decellularized fetal bone marrow is useful as a cell culture scaffold with bone marrow ECM and structure.

利益相反に関する開示

The authors declare no conflicts of interest associated with this manuscript.

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