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A genotyping method for discrimination between Saccharomyces cerevisiae and Saccharomyces paradoxus from natural environment

##article.authors##

  • Fuka Sawaguchi Faculty of Food Industry, Niigata Agro-Food University
  • Yuki Kobashi Faculty of Food Industry, Niigata Agro-Food University
  • Takashi Kuribayashi Faculty of Food Industry, Niigata Agro-Food University https://orcid.org/0000-0002-0719-6005
  • Hina Satone Faculty of Food Industry, Niigata Agro-Food University

DOI:

https://doi.org/10.51094/jxiv.1287

キーワード:

screening、 Saccharomyces cerevisiae、 Saccharomyces paradoxus

抄録

In this study, we developed a straightforward technique for differentiating between Saccharomyces cerevisiae and Saccharomyces paradoxus, thereby facilitating the isolation of S. cerevisiae from natural environments. Our findings suggest that this method is easy, quick, and beneficial for producing fermented foods and beverages using wild S. cerevisiae.

利益相反に関する開示

The authors declare no conflict of interest. All experiments complied with the current laws of the country, in which they were performed. This study was presented orally at the 69th Annual Meeting of the Mycological Society of Japan (E-12; 18th, May, 2025

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引用文献

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Hashim, H. O., & Al-Shuhaib, M. B. S. (2019). Exploring the potential and limitations of PCR-RFLP and PCR-SSCP for SNP detection: A review. Journal of Applied Biotechnology Reports, 6(4), 137-144.

Kitagaki, H., & Kitamoto, K. (2013). Breeding research on sake yeasts in Japan: history, recent technological advances, and future perspectives. Annual Review of Food Science and Technology, 4(1), 215-235.

Nikulin, J., Vidgren, V., Krogerus, K., Magalhães, F., Valkeemäki, S., Kangas-Heiska, T., & Gibson, B. (2020). Brewing potential of the wild yeast species Saccharomyces paradoxus. European Food Research and Technology, 246, 2283-2297.

Sampaio, J. P., & Gonçalves, P. (2008). Natural populations of Saccharomyces kudriavzevii in Portugal are associated with oak bark and are sympatric with S. cerevisiae and S. paradoxus. Applied and Environmental Microbiology, 74(7), 2144-2152.

Sniegowski, P. D., Dombrowski, P. G., & Fingerman, E. (2002). Saccharomyces cerevisiae and Saccharomyces paradoxus coexist in a natural woodland site in North America and display different levels of reproductive isolation from European conspecifics. FEMS Yeast Research, 1(4), 299-306.

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投稿日時: 2025-06-02 15:02:58 UTC

公開日時: 2025-06-04 08:36:12 UTC
研究分野
農学・食品科学