DOI: 10.6620/ZS.2023.62-21
プレプリント / バージョン1

New optional intermediate hosts of Mothocya parvostis (Isopoda: Cymothoidae): Juveniles of the cobaltcap silverside Hypoatherina tsurugae (Atheriniformes: Atherinidae) and the yellowfin seabream Acanthopagrus latus (Perciformes: Sparidae)


  • Fujita, Hiroki Graduate School of Integrated Sciences for Life, Hiroshima University
  • Kawai, Kentaro Graduate School of Integrated Sciences for Life, Hiroshima University
  • Deville, Diego Graduate School of Integrated Sciences for Life, Hiroshima University
  • Umino, Tetsuya Graduate School of Integrated Sciences for Life, Hiroshima University




Life cycle、 Manca、 New host record、 Parasitic cymothoid、 Prevalence


Mothocya parvostis (Isopoda: Cymothoidae) is a crustacean that infests the opercular cavities of fishes. Its main definitive host is Japanese halfbeak Hyporhamphus sajori. However, M. parvostis also infests black sea bream Acanthopagrus schelgelii as an optional intermediate host. Hence, to infest not only definitive hosts but also optional intermediate hosts; this is important for understanding the life history of Cymothoidae, and further information should be obtained. In this study, 20 and 144 cymothoids (mancae and juveniles, respectively) were collected from 129 cobaltcap silversides Hypoatherina tsurugae and 494 yellowfin seabreams Acanthopagrus latus. Molecular analysis of the cytochrome c oxidase subunit I gene and 16S rRNA genes revealed that cymothoid mancae and juveniles from the two fish species were identified to be M. parvostis. Hypoatherina tsurugae and A. latus juveniles were optional intermediate hosts of M. parvostis. Mothocya parvostis mancae infested juveniles of both species just after metamorphosis, grew with the host, and detached from the fish as juveniles continued growing. The parasitic status of M. parvostis in the three optional intermediate hosts indicated that M. parvostis likely reproduced from June to December, and different optional intermediate hosts were used depending on the time of year in Hiroshima Bay. Therefore, a parasitic strategy involving optional intermediate hosts might increase the infestation success of M. parvostis to H. sajori.

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Abol-Munafi AB, Ueda S. 1994. The gonadal cycle of the yellowfin porgy, Acanthopagrus latus (Houttuyn) reared in the net cage at Tosa Bay, Japan. Aquac Sci 42(1):135–144. doi:10.11233/aquaculturesci1953.42.135.

Aneesh PT, Sudha K, Helna AK, Anilkumar G. 2016. Mothocya renardi (Bleeker, 1857) (Crustacea: Isopoda: Cymothoidae) parasitizing Strongylura leiura (Bleeker) (Belonidae) off the Malabar coast of India: re-description, occurrence and life cycle. Syst Parasitol 93:583–599. doi:10.1007/s11230-016-9646-8.

Baillie C, Welicky RL, Hadfield KA, Smit NJ, Mariani S, D Beck RM. 2019. Hooked on you: shape of attachment structures in cymothoid isopods reflects parasitic strategy. BMC Evol Biol 19(1):207. doi: 10.1186/s12862-019-1533-x

Bello G, Vaglio A, Piscitelli G. 1997. The reproductive cycle of Mothocya epimerica (Isopoda: Cymothoidae) a parasite of the sand smelt, Atherina boyeri (Osteichthyes: Atherinidae), in the Lesina Lagoon, Italy. J Nat Hist 31(7):1055–1066. doi:10.1080/00222939700770551

Bleeker P. 1854. Faunae Ichthyologicae japonicae. Species Novae. Natuurwet Tijdschr Ned Indie 6:395–426.

Boyko CB, Bruce NL, Hadfield KA, Merrin KL, Ota Y, Poore GCB, Taiti S, Schotte M, Wilson GDF. (eds), 2008 onwards. World Marine, Freshwater and Terrestrial Isopod Crustaceans database. Cymothoidae Leach, 1818. Accessed through: World Register of Marine Species. Available from http://www.marinespecies.org/aphia.php?p=taxdetails&id=118274. Accessed 8 May 2022.

Bruce NL. 1986. Revision of the isopod crustacean genus Mothocya Costa, in Hope, 1851 (Cymothoidae: Flabellifera), parasitic on marine fishes. J Nat Hist 20:1089–1192. doi:10.1080/00222938600770781

Bruce NL. 1987. Australian Pleopodias Richardson, 1910, and Anilocra Leach, 1818 (Isopoda: Cymothoidae), crustacean parasites of marine fishes. Rec Aust Mus 39:85–130. doi:10.3853/j.0067-1975.39.1987.166

Bruce NL. 1987. Australian species of Nerocila Leach, 1818, and Creniola n. gen. (Isopoda: Cymothoidae), crustacean parasites of marine fishes. Rec Aust Mus 39:355–412. doi:10.3853/j.0067-1975.39.1987.174

Dana JD. 1852. On the classification of the Crustacea Choristopoda or Tetradecapoda. Am J Sci 2:297–316.

Fogelman RM, Grutter AS. 2008. Mancae of the parasitic cymothoid isopod, Anilocra apogonae: early life history, host-specificity, and effect on growth and survival of preferred young cardinal fishes. Coral Reefs 27:685–693. doi:10.1007/s00338-008-0379-2

Folmer O, Black M, Hoeh W, Lutz R, Vrijenhoek R. 1994. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Mol Mar Biol Biotech 3:294–299.

Fujita H, Kawai K, Taniguchi R, Tomano S, Sanchez G, Kuramochi T, Umino T. 2020. Infestation of the parasitic isopod Mothocya parvostis on juveniles of the black sea bream Acanthopagrus schlegelii as an optional intermediate host in Hiroshima Bay. Zool Sci 37:544–553. doi:10.2108/zs190147

Fujita H, Umino T, Saito N. 2021. Molecular identification of the aegathoid stage of Anilocra clupei (Isopoda: Cymothoidae) parasitizing sweeper Pempheris sp. (Perciformes: Pempheridae). Crustacean Res 50:29–31. doi:10.18353/crustacea.50.0_29

Fujita H. Infestation of the aegathoid stage of Anilocra clupei (Isopoda: Cymothoidae) on a Japanese halfbeak Hyporhamphus sajori (Beloniformes: Hemiramphidae) collected from Japan. Cancer 31. (in press) (in Japanese with English abstract)

Hata H, Sogabe A, Tada S, Nishimoto R, Nakano R, Kohya N, Takeshima H, Kawanishi R. 2017. Molecular phylogeny of obligate fish parasites of the family Cymothoidae (Isopoda, Crustacea): evolution of the attachment mode to host fish and the habitat shift from saline water to freshwater. Mar Biol 164:1–15. doi:10.1007/s00227-017-3138-5

Hatai K, Yasumoto S. 1980. A parasitic isopod, Irona melanosticta isolated from the gill chamber of fingerlings of cultured yellowtail, Seriola quinqueradiata. Bull Nagasaki Pref Institute Fish 6:87–96. (In Japanese with English title)

Hesp SA, Potter IC, Hall NG. 2004. Reproductive biology and protandrous hermaphroditism in Acanthopagrus latus. Environ Biol Fish 70(3):257–272. doi:10.1023/B:EBFI.0000033344.21383.00

Houttuyn, M. (1782). Beschrijving van eenige Japanse visschen en andere zee-schepzelen (Vol. 1).

Iwatsuki, Y. 2013. Review of the Acanthopagrus latus complex (Perciformes: Sparidae) with descriptions of three new species from the Indo-West Pacific Ocean. J Fish Biol 83(1):64–95. doi:10.1111/jfb.12151

Jones CM, Miller TL, Grutter AS, Cribb TH. 2008. Natatory-stage cymothoid isopods: description, molecular identification and evolution of attachment. Int J Parasitol 38(3–4):477–491. doi: 10.1016/j.ijpara.2007.07.013

Jordan DS, Starks EC. 1901. A review of the atherine fishes of Japan. Proc US Natl Mus 24:199–206.

Kawai K, Fujtia H, Umino T. 2019. Horizontal distribution and annual fluctuations in abundance of settled juveniles of the black sea bream Acanthopagrus schlegelii in Hiroshima Bay, Japan. Bull Hiroshima Univ Mus 11:1–5.

Kawai K, Fujita H, Sanchez G, Furusawa S, Umino T. 2020. Estimating the spawning season of black sea bream Acanthopagrus schlegelii in Hiroshima Bay, Japan, from temporal variation in egg density. Fisheries Sci 86(4):645–653. doi:10.1007/s12562-020-01433-1

Kawai K, Fujita H, Sanchez G, Umino T. 2021. Oyster farms are the main spawning grounds of the black sea bream Acanthopagrus schlegelii in Hiroshima Bay, Japan. PeerJ 9:e11475. doi:10.7717/peerj.11475

Kawai K, Okazaki R, Tomano S, Umino T. 2017. DNA identification and seasonal changes of pelagic fish eggs in Hiroshima Bay. Nippon Suisan Gakk 83:215–217 (in Japanese with English abstract) doi:10.2331/suisan.16-00069

Kawanishi R, Sogabe A, Nishimoto R, Hata H. 2016. Spatial variation in the parasitic isopod load of the Japanese halfbeak in western Japan. Dis Aquat Organ 122:13–19. doi:10.3354/dao03064

Leach WE. 1818. Cymothoadées. In Cuvier, F. (Ed), Dictionnaire des Sciences Naturelles, Vol. 12. Paris, pp. 338–354.

Mori K, Kimura S, Tsukamoto Y, Kohno Y, Yoshida M. 1988. Growth of the atherinid fish Hypoatherina tsurugae in Ago Bay, Central Japan. Aquac Sci 36(2):87–90. (in Japanese) doi:10.11233/aquaculturesci1953.36.87

Nagasawa K. 2020. Mothocya parvostis (Isopoda: Cymothoidae) parasitic on Japanese halfbeak, Hyporhamphus sajori, in the central Seto Inland Sea, Japan, with a brief summary of the hosts, geographical distribution, and pathogenic effects of the isopod. Nature of Kagoshima 47:51–57.

Nishida Y. 2022. Acanthopagrus latus. In: Kondo Y, Ohtsuka S, Sato M. (eds) Life in the tidal flats of Hachi-no-higata: the well-preserved natural ecosystem at Takehara in the Seto Inland Sea, Japan, 1st edn. NextPublishing Authors Press, Tokyo. p. 46. (in Japanese)

Okulewicz A, Perec-Matysiak A, Buńkowska K, Hildebrand J. 2012. Toxocara canis, Toxocara cati and Toxascaris leonina in wild and domestic carnivores. Helminthologia 49(1):3–10. doi:10.2478/s11687-012-0001-6

Saito N, Fujita H. Morphological description and molecular barcoding of the aegathoid stage of Nerocila japonica (Crustacea: Isopoda: Cymothoidae) infesting red seabream Pagrus major. Crustacean Res 51. (in press)

Saitou N, Nei M. 1987. The neighbor-joining method: A new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425. doi:10.1093/oxfordjournals.molbev.a040454

Simon C, Frati F, Beckenbach A, Crespi B, Liu H, Floors P. 1994. Evolution, weighting, and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved polymerase chain reaction primers. Ann Entomol Soc Am 87:651–701. doi:10.1093/aesa/87.6.651

Smit NJ, Bruce NL, Hadfield KA. 2014. Global diversity of fish parasitic isopod crustaceans of the family Cymothoidae. Int J Parasitol Parasites Wildl 3:188–197. doi:10.1016/j.ijppaw.2014.03.004

Temminck CJ, Schlegel H. 1846. Pisces. Fauna Japonica, sive descriptio animalium quae in itinere per Japoniam suscepto annis 1823-30 collegit, notis observationibus et adumbrationibus illustravit P. F. de Siebold, Parts 10–14, 173–269.

Temminck, C. J. and H. Schlegel. 1845. In: Temminck and Schlegel 1843. Fauna Japonica, sive description animalium quae in itinere per Japoniam. Parts 7–9:113–172.

Toyobo. 2012. Special feature on PCR protocols by material and application. https://lifescience.toyobo.co.jp/upload/upld99/feature/pcr99fe01.pdf. Accessed 26 May 2022. (in Japanese)

Tran TT, Tran HD, Kinoshita I. 2019. Simultaneous and sympatric occurrence of early juveniles of Acanthopagruslatus and A. schlegelii (Sparidae) in the estuary of northern Vietnam. Limnology 20(3):321–326. doi:10.1007/s10201-019-00581-3

Tsukamoto Y, Kimura S. 1993. Development of laboratory-reared eggs, larvae and juveniles of the atherinid fish, Hypoatherina tsurugae, and comparison with related species. Jpn J Ichthyol 40(2):261–267. doi:10.11369/jji1950.40.261

Yamauchi T. 2016. Cymothoid isopods (Isopoda: Cymothoidae) from fishes in Japanese waters. Cancer 25:113–119. (in Japanese) doi:10.18988/cancer.25.0_113



投稿日時: 2022-06-14 09:52:07 UTC

公開日時: 2022-06-16 01:25:32 UTC