Controlled 10°C Preservation with EP-TU Solution Attenuates Early Oxygenation Impairment in a Rat Lung Transplantation Model: 10°C EP-TU Lung Preservation

Kazunori Ueda; Watanabe, Yui; Naoya Ishida; Sho Murai; Hideki Masaki; Chikara Sakai; Tatsuaki Watanabe; Takashi Hirama; Hisashi Oishi; Sakiko Kumata; Ken Onodera; Hirotsugu Notsuda; Takaya Suzuki; Yoshinori Okada

doi:10.51094/jxiv.6741

##article.authors##

Kazunori Ueda Department of Thoracic Surgery, Institute of Development, Aging and Cancer, Tohoku University, Sendai, Japan
Watanabe, Yui Tohoku University
Naoya Ishida Department of Thoracic Surgery, Institute of Development, Aging and Cancer, Tohoku University, Sendai, Japan
Sho Murai Department of Thoracic Surgery, Institute of Development, Aging and Cancer, Tohoku University, Sendai, Japan
Hideki Masaki Stem Cell Therapy Laboratory, Institute of Integrated Research, Institute of Science Tokyo, Tokyo, Japan
Chikara Sakai Department of Thoracic Surgery, Institute of Development, Aging and Cancer, Tohoku University, Sendai, Japan
Tatsuaki Watanabe Department of Thoracic Surgery, Institute of Development, Aging and Cancer, Tohoku University, Sendai, Japan
Takashi Hirama Department of Thoracic Surgery, Institute of Development, Aging and Cancer, Tohoku University, Sendai, Japan
Hisashi Oishi Department of Thoracic Surgery, Institute of Development, Aging and Cancer, Tohoku University, Sendai, Japan
Sakiko Kumata Department of Thoracic Surgery, Institute of Development, Aging and Cancer, Tohoku University, Sendai, Japan
Ken Onodera Department of Thoracic Surgery, Institute of Development, Aging and Cancer, Tohoku University, Sendai, Japan
Hirotsugu Notsuda Department of Thoracic Surgery, Institute of Development, Aging and Cancer, Tohoku University, Sendai, Japan
Takaya Suzuki Department of Thoracic Surgery, Institute of Development, Aging and Cancer, Tohoku University, Sendai, Japan
Yoshinori Okada Department of Thoracic Surgery, Institute of Development, Aging and Cancer, Tohoku University, Sendai, Japan

DOI:

https://doi.org/10.51094/jxiv.6741

キーワード:

Ischemia-reperfusion injury、 Lung preservation、 Lung transplantation、 Primary graft dysfunction、 Rat lung transplantation

抄録

Static cold storage at approximately 4°C remains a simple and widely used method for donor lung preservation; however, the optimal temperature for prolonged lung preservation remains uncertain. Controlled 10°C preservation has recently attracted renewed interest, but its feasibility with EP-TU lung preservation solution, an extracellular-type phosphate-buffered solution developed at Tohoku University, has not been clarified. This study evaluated the feasibility and early graft function of controlled 10°C preservation using EP-TU solution compared with conventional 4°C preservation in a rat orthotopic left lung transplantation model. Male Lewis rat donor lungs were flushed with EP-TU solution and preserved for 18 hours at either 4°C or 10°C (n = 4 per group). Orthotopic left lung transplantation was then performed using the cuff technique, followed by 2 hours of reperfusion. After reperfusion, the pulmonary venous oxygen partial pressure/fraction of inspired oxygen ratio was significantly higher in the 10°C group than in the 4°C group [153.5 mmHg (109.0–183.1) vs. 65.95 mmHg (27.0–83.4), p = 0.0286]. The wet-to-dry weight ratio tended to be lower in the 10°C group than in the 4°C group [7.240 (5.3988–7.9452) vs. 8.506 (7.5072–9.3987), p = 0.0571]. Histologic lung injury score, TUNEL-positive cell ratio, and inflammatory cytokine messenger RNA expression did not differ significantly between the groups. Controlled 10°C preservation using EP-TU solution was feasible and attenuated early impairment of graft oxygenation compared with conventional 4°C preservation after prolonged static storage, without significant differences in these injury markers. These findings support further investigation of EP-TU-based 10°C preservation as a strategy for safe and prolonged lung preservation.

利益相反に関する開示

The authors declare that they have no conflicts of interest related to this study.

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引用文献

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Controlled 10°C Preservation with EP-TU Solution Attenuates Early Oxygenation Impairment in a Rat Lung Transplantation Model

10°C EP-TU Lung Preservation