Selective Hydrolysis of Nectar Phytochemical Monoglucosides during Honey Maturation: Possible Role of Honeybee Crop-derived Microbial β-glucosidases

Kato, Yoji; Yagi, Erina; Nakai, Hayato; Kikkawa, Natsumi; Umeda, Miyu; Okada, Ryuichi; Sakura, Midori; Nishikawa, Miyu; Ikushiro, Shinichi; Nakanishi, Toyofumi; Niwa, Toshio

doi:10.51094/jxiv.5275

##article.authors##

Kato, Yoji School of Human Science and Environment, University of Hyogo
Yagi, Erina School of Human Science and Environmen, University of Hyogo
Nakai, Hayato School of Human Science and Environment, University of Hyogo
Kikkawa, Natsumi School of Human Science and Environment, University of Hyogo
Umeda, Miyu School of Human Science and Environment, University of Hyogo
Okada, Ryuichi Department of Biology, Kobe University https://researchmap.jp/7000021642
Sakura, Midori Department of Biology, Kobe University https://researchmap.jp/7000004009
Nishikawa, Miyu Faculty of Agriculture, University of Miyazaki https://researchmap.jp/mnisikawa
Ikushiro, Shinichi Department of Biotechnology, Faculty of Engineering, Toyama Prefectural University https://researchmap.jp/read0021300
Nakanishi, Toyofumi Faculty of Medical Sciences, Shubun University https://researchmap.jp/read0060579
Niwa, Toshio Faculty of Health and Nutrition, Shubun University https://researchmap.jp/RM-17

DOI:

https://doi.org/10.51094/jxiv.5275

キーワード:

phytochemical、 nectar、 honey、 glycoside、 glucosidase、 honeybee-derived bacteria

抄録

This study aimed to explore the mechanisms underlying the selective loss of nectar phytochemical monoglucosides in honey. Leptosperin (methyl syringate 4-O-β-D-gentiobioside) and methyl syringate 4-O-β-D-glucoside (MSYR-Glc) were first selected as representatives of nectar-derived glycosides. When glycosides were mixed with either a 5% honey solution or a protein-rich honey extract, MSYR-Glc was converted to MSYR, whereas leptosperin remained unaffected. This activity was heat-labile, suggesting enzymatic involvement. Other phytochemical monoglucosides were also cleaved, but one rhamnoside, glucuronides, and diglycosides were not. The cleavage reaction was slow in the 72% sugar solution, comparable to that of mature honey, but proceeded at a 40% sugar concentration, corresponding to flower nectars. β-Glucosidase-positive Bifidobacterium species isolated from honeybee crops showed substrate specificities similar to the honey proteins. The selective hydrolysis of nectar-derived monoglucosides may be mediated by microbe-derived β-glucosidase activity during the early stages of honey maturation.

利益相反に関する開示

The authors declare no conflicts of interest related to this study.

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