Molecular cloning, recombinant protein expression and purification of a novel carboxylesterase from Liposcelis bostrychophila
キーワード:Liposcelis bostrychophila、 carboxylesterase、 insecticide、 synergist、 booklouse
Booklice are tiny insect pests commonly found in an indoor environment and characterized by extremely high proliferation ability. Booklouse contamination in stored foods causes serious food loss; therefore, they are recognized as so-called stored-food pests. Furthermore, since booklice in house dust have been reported to be a potent allergen, it should be also considered that the accidental ingestion of booklice possibly causes allergic symptom. Therefore, safe extermination of booklice would contribute to the solution of both food-loss and allergic concerns. Organophosphates (OPs) are compounds generally used as harmless insecticides; nonetheless, the cases of OP poisoning are sometimes reported worldwide. Therefore, considering usage for stored food products, OP doses should be low as far as possible. It is known that carboxylesterase-mediated inactivation of OPs decreases the efficacy of OPs, suggesting that an inhibitor of carboxylesterase could be a synergist to reduce the dose of OP. Herein, I report the molecular cloning and characterization of a novel carboxylesterase highly expressed in a representative species of indoor booklice, Liposcelis bostrychophila.
Mbow C, Rosenzweig C, Barioni LG, Benton TG, Herrero M, Krishnapillai M, Liwenga E, Pradhan P, Rivera-Ferre MG, Sapkota T, Tubiello FN, Xu Y. 2019: Food Security. In: Climate Change and Land: an IPCC special report on climate change, desertification, land degradation, sustainable land management, food security, and greenhouse gas fluxes in terrestrial ecosystems.
Kumar D, Kalita P. Reducing Postharvest Losses during Storage of Grain Crops to Strengthen Food Security in Developing Countries. Foods 2017;6:8.
Campbell JF, Arthur FH, Mullen MA. Insect management in food processing facilities. Adv Food Nutr Res. 2004;48:239-95.
Nayak MK, Collins PJ, Throne JE, Wang JJ. Biology and management of psocids infesting stored products. Annu Rev Entomol. 2014;59:279-97.
Kučerová Z. Weight losses of wheat grain caused by psocid infestation (Liposcelis bostrychophila: Liposcelididae: Psocoptera). Plant Protect. Sci. 2002;38:103-107.
Fukutomi Y, Kawakami Y, Taniguchi M, Saito A, Fukuda A, Yasueda H, Nakazawa T, Hasegawa M, Nakamura H, Akiyama K. Allergenicity and cross-reactivity of booklice (Liposcelis bostrichophila): a common household insect pest in Japan. Int Arch Allergy Immunol. 2012;157:339-348.
Ishibashi O, Sakuragi K, Fukutomi Y, Kawakami Y, Kamata Y, Sakurai M, Nakayama S, Uchiyama H, Kobayashi H, Kojima H, Inui T. Lip b 1 is a novel allergenic protein isolated from the booklouse, Liposcelis bostrychophila. Allergy 2017;72:918-926.
Clemmons EA, Taylor DK. Booklice (Liposcelis spp.), Grain Mites (Acarus siro), and Flour Beetles (Tribolium spp.): 'Other Pests' Occasionally Found in Laboratory Animal Facilities. J Am Assoc Lab Anim Sci. 2016;55:737-743.
Collins PJ, Nayak MK, Kopittke R. Residual efficacy of four organophosphate insecticides on concrete and galvanized steel surfaces against three liposcelid psocid species (Psocoptera: Liposcelidae) infesting stored products. J Econ Entomol. 2000;93:1357-1363.
Ding W, Zhao ZM, Wang JJ, Tao HY, Zhang, YQ. The relationship between resistance to controlled atmosphere and insecticides of Liposcelis bostrychophila Badonnel (Psocoptera:Liposcelididae). Scientia Agricultura Sinica. 2004;37:1308–1315.
Khan S, Uddin MN, Rizwan M, Khan S, Uddin MN, Rizwan M, Khan W, Farooq M, Sattar Shah A, Subhan F, Aziz F, Rahman KU, Khan A, Ali S, Muhammad M. Mechanism of Insecticide Resistance in Insects/Pests. Polish Journal of Environmental Studies. 2020;29:2023-2030.
Boyer S, Zhang H, Lempérière G. A review of control methods and resistance mechanisms in stored-product insects. Bull Entomol Res. 2012;102:213-229.
Correy GJ, Zaidman D, Harmelin A, Carvalho S, Mabbitt PD, Calaora V, James PJ, Kotze AC, Jackson CJ, London N. Overcoming insecticide resistance through computational inhibitor design. Proc Natl Acad Sci USA. 2019;116:21012-21021.
Ashour MB, Gee SJ, Hammock BD. Use of a 96-well microplate reader for measuring routine enzyme activities. Anal Biochem. 1987;166:353-360.
投稿日時: 2022-10-07 04:35:55 UTC
公開日時: 2022-10-11 10:43:20 UTC
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