Two complementary forest-originated Gigaspora spp. shifted shoot-to-root ratio for growth improvement in Cryptomeria japonica seedlings: Gigaspora AMF shifted shoot-to-root ratio in Cryptomeria japonica

DJOTAN, Akotchiffor Kevin Geoffroy; Yosuke Matsuda; Norihisa Matsushita; Kenji Fukuda

doi:10.51094/jxiv.1773

##article.authors##

DJOTAN, Akotchiffor Kevin Geoffroy Mie University, Graduate School of Bioresources, Department of Sustainable Resources Sciences https://orcid.org/0000-0002-3726-9826
Yosuke Matsuda Mie University, Graduate School of Bioresources, Department of Sustainable Resources Sciences https://orcid.org/0000-0002-7001-3101
Norihisa Matsushita The University of Tokyo, Graduate School of Agricultural and Life Sciences, Department of Forest Sciences, Laboratory of Forest Botany
Kenji Fukuda The University of Tokyo, Graduate School of Agricultural and Life Sciences, Department of Forest Sciences, Laboratory of Forest Botany

DOI:

https://doi.org/10.51094/jxiv.1773

キーワード:

Forest AMF、 Inoculation assays、 Gigaspora rosea、 Gigaspora margarita、 AMF cohabitation

抄録

Arbuscular mycorrhizal fungi (AMF) are potential bioinoculants to grow healthy plants in healthy soils. However, most available AMF isolates originated from nonforest environments and AMF contributions to tree seedlings remain unclear. Here, our objective was to clarify the functions of forest-inhabiting AMF in tree seedling production. To achieve this, we first identified two Gigaspora AMF (LFB-4 and LFB-A1) previously isolated from Cryptomeria japonica (Cupressaceae) trees and characterized them using morphological and molecular analyzes. We then carried out inoculation assays to clarify the cohabitation and functions of LFB-4 and LFB-A1 in C. japonica seedlings. We identified LFB-4 and LFB-A1 as Gigaspora rosea and Gigaspora margarita, respectively. They produced spores inside host root cells, simultaneously developed multiple germ tubes during germination, and showed presymbiotic sporulation. LFB-A1 produces spores as large as 500 µm in diameter. LFB-4 and LFB-A1 were both beneficial AMF with different functions and complementary effects on the growth of C. japonica seedlings. In their cohabitation, while LFB-4 boosted water uptake and height growth, LFB-A1 improved biomass production. Together, they encouraged carbon release into the soil and increased the shoot-to-root biomass ratio for faster seedling growth, although without increasing root colonization. We concluded that, despite erratic root colonization, G. rosea and G. margarita worked synergistically to improve the growth of C. japonicaseedlings by modulating root development, likely for carbon acquisition. Root colonization assessed by microscopy or metabarcoding may overlook AMF functions.

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The authors declare that they have no conflicts of interest

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Two complementary forest-originated Gigaspora spp. shifted shoot-to-root ratio for growth improvement in Cryptomeria japonica seedlings

Gigaspora AMF shifted shoot-to-root ratio in Cryptomeria japonica