Forest thinning has little effect on the spatiotemporal dynamics of arbuscular mycorrhizal fungi community in Cryptomeria japonica roots

DJOTAN, Akotchiffor Kevin Geoffroy; Norihisa Matsushita; Yosuke Matsuda; Kenji Fukuda

doi:10.51094/jxiv.1156

##article.authors##

DJOTAN, Akotchiffor Kevin Geoffroy Mie University, Graduate School of Bioresources, Department of Sustainable Resource Sciences, Laboratory of Forest Mycology https://orcid.org/0000-0002-3726-9826
Norihisa Matsushita The University of Tokyo, Graduate School of Agricultural and Life Sciences, Department of Forest Sciences, Laboratory of Forest Botany
Yosuke Matsuda Mie University, Graduate School of Bioresources, Department of Sustainable Resource Sciences
Kenji Fukuda The University of Tokyo, Graduate School of Agricultural and Life Sciences, Department of Forest Sciences, Laboratory of Forest Botany https://orcid.org/0000-0002-9980-3107

DOI:

https://doi.org/10.51094/jxiv.1156

キーワード:

Forest management、 Thinning、 AMF ecology、 Metabarcoding、 Spatiotemporal distribution

抄録

Stand thinning affects forest physiognomy above- and belowground, but we ignore how it affects root-associated arbuscular mycorrhizal fungi (AMF) in trees. We aimed to investigate how stand thinning affects the dynamics of the AMF community in trees. Root and soil samples of twenty selected Cryptomeria japonica (Cupressaceae) trees were collected every August from 2021 to 2023 at four microsites with and without stand thinning, established in nearly 1 km²of a C. japonica plantation in central Japan. We amplified ~550 bp of a partial small subunit of fungal ribosomal DNA and amplicons were sequenced with Illumina Miseq to investigate the root AMF community composition. Soil pH, total C, N, and C/N were also measured. As a result, we observed significant (1) spatial variation in pH, total C, and N; (2) spatiotemporal dynamics in C/N, AMF richness, and Shannon index increasing from the first year to the second, then decreasing down to the initial status from the second year to the third; and (3) spatial variation in the AMF community composition mainly driven by soil pH, total C, N, and C/N; all irrespective of stand-thinning treatment. A light thinning does not suddenly affect the soil properties that influence AMF distribution, and thus the root AMF community of spared trees remains unchanged till two years post-thinning. Our findings warned that stochasticity should be considered when analyzing AMF’s response to treatments in long-term studies.

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The authors declare that they have no conflicts of interest.

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